DNA

Part:BBa_K4747012:Design

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


PA0727


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 799
    Illegal PstI site found at 286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 799
    Illegal PstI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 799
    Illegal BamHI site found at 741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 799
    Illegal PstI site found at 286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 586
    Illegal EcoRI site found at 799
    Illegal PstI site found at 286
    Illegal NgoMIV site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To construct vectors for aiiA/ytnP/wspF/yhjH expression


Source

PA0727 is a Pf replication initiator protein located within the cytoplasmic membrane of P. aeruginosa PAO1.

References